奥曲肽抑制肝星状细胞增殖及细胞外基质合成

• 作 者: 王志荣 陈锡美 李定国 黄新 魏红山 展玉涛 汪余勤 陆汉明
• 机 构: 同济大学附属同济医院消化内科,同济大学附属同济医院消化内科,上海第二医科大学新华医院消化内科,上海第二医科大学新华医院消化内科,上海第二医科大学新华医院消化内科,上海第二医科大学新华医院消化内科,上海
• 出 处: 中国病理生理杂志
• 发表日期: 2004年02期
• 关键词: 奥曲肽 肝星状细胞 细胞外基质 增殖

目的 :探讨奥曲肽对肝星状细胞 (HSC)增殖与细胞外基质 (ECM)合成的影响。方法 :采用胶原酶二步原位灌注法分离、培养大鼠HSC ,并分别给予转化生长因子 1(TGFβ1) (2 5 μg·L-1)、奥曲肽 (Oct) (0 0 1- 10μg·L-1)或TGFβ1(2 5 μg·L-1) +Oct (0 0 1- 10mg·L-1)干预 ,分别用MTT法、[3 H]-TdR和 [3 H]-脯氨酸掺入法及放射免疫法检测各处理组HSC增殖及ECM合成水平。结果 :Oct能不同程度抑制HSC [3 H]-TdR掺入和增殖 ;能够显著抑制体外培养HSC [3 H]-脯氨酸掺入 ,降低上清液透明质酸 (HA)、层粘连蛋白 (LN)和IV型胶原 (CIV)水平 ;TGFβ1能够诱导HSC表达ECM上调 ,Oct能够阻断TGFβ1对HSC的调控作用。 结论 :Oct能够有效地抑制HSC增殖及ECM合成与分泌。

Objective: To investigate the effects of octreotide on the proliferation of hepatic stellate cells (HSC) and the synthesis of extracellular matrix (ECM). METHODS: Rat HSCs were isolated and cultured by collagenase two-step in situ perfusion. TGFβ1 (25 μg · L-1), octreotide (0 0 1- 10 μg · L MTT method, [3 H] -TdR and [3 H] -proline incorporation were used to investigate the effects of TGFβ1 (25 μg · L -1) and Oct (0 0 1 -10 mg · L -1) Addition and radioimmunoassay were used to detect HSC proliferation and ECM synthesis in each treatment group. Results: Oct inhibited the incorporation and proliferation of [3 H] -TdR in HSC to varying degrees, and inhibited the incorporation of [3 H] -proline in HSC significantly and decreased the content of hyaluronic acid (HA), laminin (LN) and type IV collagen (CIV). TGFβ1 can up-regulate the expression of ECM in HSC, and Oct can block the regulation of TGFβ1 on HSC. Conclusion: Oct can effectively inhibit HSC proliferation and ECM synthesis and secretion.