论文部分内容阅读
利用抗人活化血小板α颗粒膜蛋白(GMP-140)特异性单克隆抗体SZ-51来检测10例体外循环不同时间段体内血小板的活化及破坏程度。体外转流中体内血小板数呈显著性下降,以转流结束时为最低;血小板表面GMP-140分子数转流开始就显著升高,转流结束至鱼精蛋白中和达高峰,术后2h恢复正常;而血浆内GMP-140的浓度,于转流结束才开始升高,术后2h达高峰,术后24至48h恢复正常;血浆内TxB_2于肝素化后及转流开始出现第一次高峰,转流结束及鱼精蛋白中和后出现第二次高峰,术后2h恢复正常;6-酮-PGF_(lα)无显著性改变;血小板因子4于转流后开始升高,转流结束达高峰,术后24h恢复正常;vWF于转流结束开始升高,术毕与术后2h为高峰,24h恢复正常,这一结果阐明了体外循环中体内血小板存在一过性的活化及破坏过程。
The activation and destruction of platelets in 10 cases of cardiopulmonary bypass were detected by SZ-51, a monoclonal antibody against human activated platelet α-granule membrane protein (GMP-140). The number of in vivo platelets decreased significantly in vitro, and the lowest was reached at the end of the commutation. The number of GMP-140 molecules on the surface of platelets increased significantly from the beginning of commutation to the peak of protamine neutralization at the end of commutation. After 2h Returned to normal; however, the concentration of GMP-140 in plasma began to increase at the end of the commutation and reached the peak at 2h after operation and returned to normal at 24 to 48h after operation. TxB_2 in plasma began to be the first Peak, end of commutation and protamine neutralization after the second peak, 2h after surgery returned to normal; 6-keto-PGF (lα) no significant change; platelet factor 4 after the start of the increase in blood flow, The peak reached the peak at 24 hours after operation, and returned to normal at 24 hours after operation. The vWF began to increase at the end of commutation, peaked at 2 hours after operation and peaked at 2 hours after operation, and returned to normal at 24h. This result illustrates the transient activation and destruction of platelets during cardiopulmonary bypass process.