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应用聚合酶链反应（ＰＣＲ）技术检测４５份急性淋巴细胞白血病（ＡＬＬ）脑脊液标本ＩｇＨ、ＴＣＲＶγＩ－Ｊγ及ＴＣＲＶδ２－Ｄδ３基因重排，从２２份标本（４８．９％）发现基因重排阳性，而用形态学方法检查，阳性率为２４．４％（１１／４５）；２２例阳性病人中的２例（９．１％）脑脊液白血病细胞基因重排类型同骨髓标本不一致，此两例均为中枢神经系统白血病（ＣＮＳＬ）复发。上述结果表明，应用ＰＣＲ技术检测ＡＬＬ脑脊液标本重排基因有助于隐匿ＣＮＳＬ诊断；克隆演化现象可能是某些单独ＣＮＳＬ复发的主要原因
PCR was used to detect the rearrangement of IgH, TCRVγI-Jγ and TCR Vδ2-Dδ3 genes in 45 CSF samples of acute lymphoblastic leukemia (ALL). Positive gene rearrangements were found in 22 samples (48.9%). The morphological examination showed that the positive rate was 24.4% (11/45); in 2 of the 22 positive patients (9.1%), the type of gene rearrangement of cerebrospinal fluid leukemia cells was inconsistent with the bone marrow specimens. Both cases were inconsistent. Recurrence of CNSL. The above results indicate that the application of PCR to detect cerebrospinal fluid specimen rearrangement gene contributes to the diagnosis of concealed CNSL; clonal evolution may be the main reason for the recurrence of certain CNSL