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Two-dimensional NMR study on the single-stranded octadeoxynucleotide,d(TT-GGGGTT)is presented.Assignments of the base protons,the H1′,H2′ and H2″ protons were madeby two-dimensional Nuclear Overhauser Enhancement Spectroscopy(NOESY)and two-dimensionalhomonuclear J-correlated Spectroscopy(COSY).The result indicates that the overall structure of thesingle-stranded d(TTGGGGTT)is a right-handed B-type helix.