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目的:探讨不同脑温状态下银杏叶提取物(GBE)对大鼠缺血脑组织γ-氨基丁酸(GABA)的影响。方法:建立大鼠大脑中动脉缺血再灌注模型,诱导目标脑温,测定轻度高温、常温、亚低温状态下各组缺血脑组织(GABA)含量。结果:(1)与常温假手术组比较,常温脑缺血对照组及常温银杏叶组GABA水平显著增高(P<0.01~0.001)。(2)与常温脑缺血对照组比较,常温银杏叶组及亚低温脑缺血组GABA水平显著增高(P<0.01~0.001)。(3)与常温银杏叶组比较,轻度高温银杏叶组GABA水平显著降低(P<0.01);亚低温脑缺血组及亚低温银杏叶组GABA水平显著增高(P<0.01~0.001)。(4)与亚低温脑缺血组比较,亚低温银杏叶组GABA水平差异无统计学意义(P>0.05)。结论:(1)脑缺血时,不同脑温可能影响GBE对GAGA水平的调节。亚低温状态下,GBE上调GABA水平的作用显著增强,可能有利于“抑制性保护”机制的建立,从而增强GBE的神经保护作用。(2)亚低温和GBE联合干预,增强GABA水平的上调,可能不是单一因素的作用,而是亚低温作用为主,两种干预因素共同协同作用的结果。
OBJECTIVE: To investigate the effect of Ginkgo biloba extract (GBE) on γ-aminobutyric acid (GABA) in ischemic brain tissue under different brain temperature conditions. METHODS: A model of middle cerebral artery ischemia and reperfusion in rats was established. The brain temperature was induced and the content of ischemic brain tissue (GABA) in mild hyperthermia, normal temperature and mild hypothermia was measured. Results: (1) Compared with the sham operation group at room temperature, GABA levels in normal temperature cerebral ischemia group and normal temperature Ginkgo biloba leaf group were significantly higher (P<0.01~0.001). (2)Compared with the normotensive cerebral ischemic control group, GABA levels in the Ginkgo biloba leaves group and the hypothermic cerebral ischemia group were significantly higher (P<0.01-0.001). (3)Compared with normal temperature Ginkgo biloba leaves group, GABA level in mild high-temperature Ginkgo biloba group was significantly decreased (P<0.01); GABA levels in mild hypothermia cerebral ischemia group and mild hypothermia Ginkgo biloba leaf group were significantly increased (P<0.01-0.001). (4) Compared with the mild hypothermia cerebral ischemia group, the GABA levels in the mild hypothermia Ginkgo biloba leaves group had no significant difference (P>0.05). Conclusion: (1) Different brain temperatures may affect the regulation of GAGA levels by GBE during cerebral ischemia. Under mild hypothermia, the effect of GBE upregulating GABA levels is significantly enhanced, which may be conducive to the establishment of “inhibitory protection” mechanism, thereby enhancing the neuroprotective effect of GBE. (2) The combination of moderate hypothermia and GBE intervention to increase the level of GABA may not be the result of a single factor, but rather the effect of mild hypothermia and the synergistic effect of the two intervention factors.