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本文主要制备载染料木黄酮的泊洛沙姆403/407混合聚合物胶束,以改善染料木黄酮的溶解度和口服生物利用度.研究采用薄膜分散法制备混合胶束,考察其形态,粒径,zeta电位,包封率和载药量;采用膜透析法考察其体外释药行为;以混悬液为对照,对混合胶束在大鼠体内的药物动力学过程进行了研究.结果显示所制备的混合胶束形态圆整,粒径均一,平均粒径为(20.31±0.43) nm,PDI为0.193,zeta电位为(-8.94±0.35) mV;包封率为和载药量分别为(90.59±0.67)％和(7.74±0.05)％;染料木黄酮在混合胶束中的溶解度达到3.80 mg/mL,比水中的溶解度提高了约130倍.混合胶束在体外呈现缓释特征,且无突释现象,但释放速度快于混悬液;大鼠灌胃混合胶束和混悬液的药动学结果显示混合胶束的AUC0-t和A UC0-∞分别为GEN混悬液的196.74％和204.62％,表明所制备的混合胶束显著地提高了染料木黄酮的溶解度和口服生物利用度.“,”In the present study,we aimed to prepare poloxamer 403/407 mixed micelles in order to improve the solubility and oral bioavailability of genistein.Genistein was incorporated in the mixed poloxamer micelles by thin-film hydration method,and its physicochemical properties,including particle size,zeta potential,entrapment efficiency and drug loading,were investigated.In vitro release of genistein from the mixed micelles was monitored by dialysis method,and pharmacokinetic study of genistein loaded mixed micelles was carried out in rats.We found that the particle size and zeta potential of mixed micelles were (20.31±0.43) nm and (-8.94±0.35) mV,with encapsulation efficiency 90.59％±0.67％ and drug loading 7.74％±0.05％.Solubility of genistein in mixed micelles reached 3.80 mg/mL,which was about 130 times higher than that in water.Genistein-loaded mixed micelles showed sustained release characteristics in vitro with no burst release phenomenon,but it was faster than suspension.The AUC0-t and AUC0-∞ of mixed micelles were 196.74％ and 204.62％ greater than that of genisein suspension,respectively.Consequently,poloxamer 403/407 mixed micelles significantly improved the solubility and oral bioavailability of genistein,which could be used as an effective drug delivery system for oral administration of poorly soluble drugs.