论文部分内容阅读
白血病是一种常见恶性肿瘤,是我国重点防治的十大恶性肿瘤之一。冬凌草甲素是一种从草药中萃取的化合物。研究表明,冬凌草甲素能够诱导肿瘤细胞凋亡,但人们对其抗癌作用的潜在分子机制还知之甚少。作者系统分析了经冬凌草甲素处理的人白血病Kasumi-1细胞的蛋白磷酸化水平变化特征,构建了蛋白位点之间磷酸化水平变化的相关性网络。不同于基因调控网络,磷酸化水平变化相关性网络呈现带状结构。网络结构显示了蛋白磷酸化信号的级联放大传递作用,以及冬凌草甲素对蛋白磷酸化水平影响的多向性。受冬凌草甲素影响而发生磷酸化水平变化的蛋白,显著富集在与控制细胞增殖、激酶结合功能、癌症相关的重要通路中。分析结果显示了冬凌草甲素影响下的蛋白磷酸化水平变化特征及其重要作用。
Leukemia is a common malignant tumor, which is one of the top ten malignant tumors in our country. Oridonin is a compound extracted from herbs. Studies have shown that oridin can induce tumor cell apoptosis, but the potential molecular mechanism of its anticancer effect is poorly understood. The authors systematically analyzed the changes of protein phosphorylation level of Kasumi-1 cells treated with oridonin, and constructed a correlation network of changes of phosphorylation level between protein sites. Unlike gene regulatory networks, the network of changes in phosphorylation shows a banded structure. The network structure shows the cascade amplification of protein phosphorylation signals and the multidirectional effect of oridonin on protein phosphorylation. Proteins that undergo phosphorylation changes under the influence of oridonin are significantly enriched in important pathways that are involved in the control of cell proliferation, kinase binding, and cancer. The results showed that the change of protein phosphorylation under the influence of oridonin and its important role.