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【摘 要】
：
黄鳍棘鲷 (Acanthopagrus latus) 是中国具有重要商业价值的海水鱼类之一，对其精子开展冷冻保存可为黄鳍棘鲷育种提供一定的技术支持，可有效预防其种质资源衰退，保持其养殖业的可持续发展。该研究以黄鳍棘鲷精子为实验材料，对其精子冷冻保存的稀释液、葡萄糖浓度、抗冻剂种类及浓度、精子与保护液稀释比例和降温程序等进行了筛选优化。结果表明，以含10 g·L−1葡萄糖、5%乙二醇的MPRS溶液与精子按1∶2的比例稀释，4 ℃平衡30 min，液氮面上5 cm熏蒸5 min，最后投入液氮保存2 h，将黄鳍
【机 构】
：
河北农业大学海洋学院,河北,秦皇岛,066003;中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室,广东,广州,510300;广东省海洋生物种业工程技术研究中心,广东,广州,
【出 处】
：
南方水产科学
【发表日期】
：
2021年1期
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