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狂犬病是由狂犬病毒引起的一种中枢神经系统急性传染病,迄今尚无特殊治疗方法,应用狂犬疫苗免疹接种预防,乃目前有效措施之一。为了进一步探讨在免疫预防狂犬病的过程中还存在的一些问题,现就我市收集的45例狂犬病免疫失败的资料作如下分析。 一、材料与方法 (一)资料来源:1988年至1989年6月济宁(11个县市)市被狂犬咬伤后发病死亡的部分完整病历;济宁市传染病防治院的抢救狂犬病人中记录的病历。 (二)血清狂犬抗体测定:ELISA法,试剂由卫生部兰州生物制品研究所生产,批号89001,血清1∶50稀释。DG-3022型酶标仪测定,波长为490nm。酶标板边缘效应带来的误差依照李树仁法消除,差异进行了校正。以P/n≥3.0为阳性,N=0.13。
Rabies is a kind of acute central nervous system infection caused by rabies virus. So far there is no special treatment. Rabies vaccination is one of the effective measures to prevent rash vaccination. In order to further explore the immunization of rabies in the process of some problems still exist, the city collected 45 cases of rabies immune failure data are analyzed as follows. I. MATERIALS AND METHODS (I) Source: Part of the complete medical records of death from Jining (11 counties) from 1988 to June 1989; records of rescue rabies from Jining City Infectious Disease Hospital Medical records. (B) serum rabies antibody determination: ELISA method, reagents from the Ministry of Health Lanzhou Institute of Biological Products, batch number 89001, serum 1:50 dilution. DG-3022 microplate reader, the wavelength of 490nm. The error caused by the edge effect of the ELISA plate is eliminated according to Li Shuren method, and the difference is corrected. Positive with P / n≥3.0, N = 0.13.