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目的探讨脑干损伤后立即早基因c-fos、c-jun的表达在脑干损伤法医学鉴定中的意义。方法采用重物自由落体式建立大鼠脑干损伤快速死亡模型,70只大鼠分为两组,实验组(40只),对照组(30只)。肉眼和HE染色观察脑干组织的形态改变,并通过原位杂交的方法检测大鼠脑干损伤部位c-fos、c-jun基因的表达情况。结果100倍光镜视野下实验组c-fos和c-jun mRNA阳性细胞数计数分别为53.62±9.84和46.85±7.43;对照组c-fos和c-junmRNA阳性细胞数分别为0.9±0.87和1.3±0.67;经统计分析,实验组c-fos、c-jun mRNA基因表达水平较对照组显著增高。结论脑干损伤后损伤部位c-fos、c-jun mRNA基因表达水平增高对脑干损伤快速死亡的法医学鉴定具有参考价值。
Objective To investigate the significance of early gene expression of c-fos and c-jun immediately after brain stem injury in forensic identification of brain stem injury. Methods Rapid death model of rat brainstem injury was established by free-falling weight. 70 rats were divided into two groups: experimental group (40 rats) and control group (30 rats). Morphological changes of brain stem were observed by naked eye and HE staining. The expression of c-fos and c-jun genes in brain stem of rats were detected by in situ hybridization. Results The number of positive cells of c-fos and c-jun mRNA in experimental group were 53.62 ± 9.84 and 46.85 ± 7.43, respectively. The number of c-fos and c-jun mRNA positive cells in control group were 0.9 ± 0.87 and 1.3 ± 0.67. By statistical analysis, the mRNA expression of c-fos and c-jun in experimental group was significantly higher than that in control group. Conclusions Increased expression of c-fos and c-jun mRNA at the injury site after brain stem injury has reference value for forensic identification of rapid death from brain stem injury.