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目的研究布洛芬对博莱霉素诱导的小鼠肺纤维化的保护作用与TGF-β和MyD88的关系.方法通过气管内滴注博莱霉素复制小鼠肺纤维化模型,治疗组行腹腔注射给予不同剂量的布洛芬.收集肺泡灌洗液用ELISA方法测定TNF-α和IL-6含量,用流式细胞仪测定细胞内ROS的水平;用Western blot方法检测肺泡灌洗液TGF-β1和MyD88的表达水平.结果与正常对照组比较,模型组支气管肺泡灌洗液ROS、TNF-α和IL-6含量显著增加(P<0.01),经布洛芬处理肺泡灌洗液中炎症因子ROS、TNF-α和IL-6明显降低(P<0.01),说明布洛芬可缓解肺纤维化所引起的ROS和炎症因子增高的现象.Western blot结果显示 TGF-β和MyD88蛋白的表达随布洛芬浓度增加而下降,20mg/kg布洛芬下降明显(P<0.01).结论布洛芬降低由博莱霉素诱导的肺纤维化细胞因子水平与下调TGF-β和MyD88表达有关.“,”Objective To study the effect of ibuprofen on the protective effect of bleomycin-induced pulmonary fibrosis in mice.Methods By copying the model of pulmonary fibrosis in mice by intratracheal instillation of bleomycin,the treatment group underwent intraperitoneal injection of different doses of ibuprofen.In collection of lavage fluid,TNF-α and IL-6 levels were analyzed by flow cytometry of intracellular and ROS levels were determined by ELISA;Lavage fluid TGF-β1 and MyD88 expression levels were detected by Western blot. Results Compared with normal control group,model group,bronchoalveolar lavage fluid ROS,TNF-α and IL-6 were significantly increased(P<0.01).Ibuprofen treatment of inflammatory factors in bronchoalveolar lavage fluid of ROS,TNF-αand of IL-6 decreased significantly(P<0.01).Ibuprofen can alleviate the phenomenon of increased ROS and inflammatory cytokines in pulmonary fibrosis caused by Western blot results showed that TGF-β and MyD88 protein expression with the increase in ibuprofen concentration decreased,20mg/kg ibuprofen significantly decreased(P<0.01).Conclusion Our study showed that ibuprofen reduce bleomycin-induced pulmonary fibrosis cytokine levels and lowered TGF-β and MyD88 expression.