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目的:研究妊娠期糖尿病小鼠胎盘表皮生长因子受体(EGFR)的表达,探讨EGFR表达与妊娠期糖尿病发病的关系。方法:采用链脲佐菌素建立妊娠期糖尿病(gestational diabetes mellitus,GDM)小鼠模型,对照组为正常妊娠小鼠,腹腔注射等量缓冲溶液。测定母鼠体重、血糖;计算胎鼠的存活率;测定胎鼠、胎盘重量,计算胎盘效率;RT-PCR、免疫组化分别测定GDM组和对照组胎盘EGFR m RNA和EGFR蛋白的表达。Pearson相关性分析用于母鼠血糖与EGFR表达的相关性分析。结果:GDM组母鼠体重和血糖均高于对照组(P<0.01);GDM组胎鼠、胎盘重量及胎盘效率均高于对照组(P<0.01);RT-PCR和免疫组化结果显示GDM组胎盘EGFR m RNA和EGFR蛋白的表达与对照组相比差异有统计学意义(P<0.01)。GDM组小鼠血糖值与其胎盘EGFR的表达具有相关性(r=0.582,P<0.05)。结论:GDM导致胎盘EGFR表达升高,EGFR并不是GDM的发病因素,EGFR是预防GDM的潜在靶点。
Objective: To study the expression of epidermal growth factor receptor (EGFR) in placenta of gestational diabetic mice and to explore the relationship between the expression of EGFR and the incidence of gestational diabetes mellitus. Methods: The gestational diabetes mellitus (GDM) mouse model was established by streptozotocin. The control group was normal pregnant mice, and the mice were injected intraperitoneally with equal volume of buffer solution. The survival rate of fetal rats was calculated. The weight of fetal rats and placenta was calculated to calculate the placental efficiency. The expression of EGFR m RNA and EGFR protein in placenta of GDM group and control group were determined by RT-PCR and immunohistochemistry respectively. Correlation Analysis of Pearson Correlation Analysis on Blood Glucose and Expression of EGFR in Female Rats. Results: The body weight and blood glucose of the GDM group were significantly higher than those of the control group (P <0.01). The weight of the fetal rat, placenta and placenta of the GDM group were higher than those of the control group (P <0.01). The results of RT-PCR and immunohistochemistry The expression of EGFR m RNA and EGFR protein in placenta of GDM group was significantly different from that in control group (P <0.01). The blood glucose level in GDM group was correlated with the expression of EGFR in placenta (r = 0.582, P <0.05). Conclusion: GDM results in increased expression of EGFR in the placenta. EGFR is not a risk factor for GDM. EGFR is a potential target for prevention of GDM.