论文部分内容阅读
目的研究人参多糖对冷应激雌性大鼠卵巢黄体细胞与颗粒细胞的作用。方法分离培养大鼠黄体细胞与颗粒细胞,按加药因素分组,以加人参多糖为实验组,分为黄体细胞实验组、颗粒细胞实验组;以不加人参多糖为对照组,分为黄体细胞对照组、颗粒细胞对照组。分别采用37、4、0℃进行冷应激实验。采用放免分析方法测定孕酮与cAMP的含量。结果37℃时,黄体细胞实验组孕酮含量为(5291.46±185.14)pmol,低于黄体细胞对照组(6573.55±326.82)pmol,颗粒细胞实验组孕酮含量为(153.16±42.87)pmol低于颗粒细胞对照组(197.42±92.46)pmol,差异均有统计学意义(P<0.05)。0℃时,黄体细胞对照组孕酮含量为(5077.37±312.47)pmol、颗粒细胞对照组孕酮含量为(149.24±25.61)pmol,分别低于37℃时黄体细胞、颗粒细胞对照组孕酮含量,差异有统计学意义(P<0.05)。黄体细胞实验组cAMP含量在37、4、0℃分别为(65.26±15.93)、(56.39±12.95)、(39.60±14.69)fmol,分别高于37、4、0℃黄体细胞对照组(31.20±17.13)、(32.76±12.88)、(20.84±13.19)fmol,颗粒细胞实验组cAMP含量在37、4、0℃分别为(296.42±27.28)、(250.69±22.29)、(239.97±17.03)fmol,分别高于37、4、0℃颗粒细胞对照组(121.15±19.96)、(189.63±10.88)、(106.10±14.05)fmol,差异均有统计学意义(P<0.05)。结论冷应激使孕马血清和人绒毛膜促性腺激素(HCG)诱导的卵巢黄体细胞与颗粒细胞孕酮和cAMP含量降低,人参多糖可抑制孕酮分泌。
Objective To investigate the effects of ginseng polysaccharide on ovarian corpus luteum and granulosa cells in cold-stressed female rats. Methods Rat luteal cells and granulosa cells were isolated and cultured. According to the factors of drug addition, the ginseng polysaccharides were used as the experimental group and divided into the test group of luteinizing cells and the experimental group of granulosa cells. The control group was divided into luteal cells without ginseng polysaccharide. Control group, granulocyte control group. Cold stress experiments were performed at 37, 4, and 0°C, respectively. Radioimmunoassay was used to determine progesterone and cAMP levels. Results At 37°C, the progesterone content in the experimental group was (5291.46 ± 185.14) pmol, which was lower than that of the luteinizing control group (6573.55 ± 326.82) pmol. Progesterone content in the granulose cell experimental group was (153.16 ± 42.87) pmol lower than that of the granules. The cell control group (197.42±92.46) pmol, the difference was statistically significant (P<0.05). At 0°C, the progesterone content in the control group was (5077.37±312.47) pmol, and the progesterone content in the granulose control group was (149.24±25.61) pmol, which was lower than that in the control group of luteinic and granular cells at 37°C. The difference was statistically significant (P<0.05). The cAMP content of the luteinized cell experimental group was (65.26±15.93), (56.39±12.95), (39.60±14.69) fmol at 37, 4, and 0°C, respectively, which was higher than that of the 37, 4, and 0° C. luteinized control group (31.20±). 17.13), (32.76±12.88), (20.84±13.19) fmol, cAMP content in granulosa cells experimental group was (296.42±27.28), (250.69±22.29), (239.97±17.03) fmol at 37, 4, and 0°C, respectively. The difference was statistically significant (P<0.05) in the control group (121.15±19.96), (189.63±10.88), and (106.10±14.05) fmol of the granulosa cells at 37, 4, and 0°C, respectively. Conclusion Cold stress can reduce the levels of progesterone and cAMP in luteal cells and granulosa cells induced by human serum and human chorionic gonadotropin (HCG). Ginseng polysaccharide can inhibit the secretion of progesterone.