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目的探讨小肠缺血再灌注损伤(IIRI)时肠道上皮增殖细胞核抗原(PCNA)的表达及其意义。方法健康成年昆明小鼠50只,随机分为空白对照组(对照组仅行单纯剖腹手术)和IIRI组,后者又分IIRI6,12,24,48h共4组,每组10只。分别于上述时段剖杀小鼠取小肠组织,常规病理切片HE染色和免疫组织化学检测PCNA的表达。结果缺血再灌注后,小肠黏膜充血、水肿、炎症细胞浸润,以24h组最显著。PCNA表达于隐窝细胞的细胞核内,主要定位于隐窝的中上部。与对照组相比,PCNA指数呈现先下降后上升的趋势;在24h组PCNA表达最少,48h开始增多。结论缺血再灌注损伤后,小肠黏膜细胞中PCNA表达先降后升的改变可能与肠道黏膜损伤后自身修复有关。
Objective To investigate the expression of proliferating cell nuclear antigen (PCNA) in intestinal ischemia-reperfusion injury (IIRI) and its significance. Methods Fifty healthy adult Kunming mice were randomly divided into blank control group (control group only laparotomy) and IIRI group, the latter divided into IIRI6, 12, 24, 48h, a total of 4 groups of 10 rats. Mice were sacrificed in the above period to take small intestine, routine histopathological HE staining and immunohistochemical detection of PCNA expression. Results After ischemia-reperfusion, intestinal mucosa congestion, edema, infiltration of inflammatory cells, the most significant 24h group. PCNA is expressed in the nucleus of crypt cells, mainly in the middle and upper part of the crypt. Compared with the control group, the PCNA index showed a trend of first decreasing and then increasing. In the 24h group, the expression of PCNA was the least, and increased at 48h. Conclusion After ischemia-reperfusion injury, the expression of PCNA in the small intestinal mucosa may first decrease and then increase, which may be related to the repair of intestinal mucosal injury.