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以SD大鼠为试验动物,饲喂高脂饲料,同时灌胃燕麦β-葡聚糖、葡萄皮超微粉碎膳食纤维和葡萄皮挤压膨化超微粉碎膳食纤维和葡萄皮可溶性膳食纤维,喂养50 d后取血,以中空纤维支撑液膜萃取-气相色谱法测定血脂试验大鼠血清的短链脂肪酸(Short-chain fatty acids, SCFA)含量,探讨不同处理葡萄皮膳食纤维对高脂膳食大鼠血清SCFA的影响。结果显示,与MC组相比,G1(超微粉碎)组对乙酸、异丁酸及异戊酸产酸能力有所提高,提高的水平分别为76.07%、194.51%和10.27%;对于丙酸、丁酸及戊酸有抑制作用,抑制水平分别为36.58%、17.68%和22.79%。G2(挤压膨化后超微粉碎)组对于产生的各酸均有抑制作用,抑制水平分别为44.27%(乙酸)、78.54%(丙酸)、60.47%(丁酸)、25.43%(异丁酸)和24.84%(异戊酸)。GS(可溶性膳食纤维)组对产乙酸能力有所提高,达127.79%,但对其他各酸有抑制作用,抑制水平分别为44.76%(丙酸)、35.37%(丁酸)、66.58%(异丁酸)、39.32%(异戊酸)和63.84%(戊酸)。
SD rats were used as experimental animals, fed with high-fat diet, orally fed β-glucan, micronized dietary fiber and grape skins, extruded extruded micronized dietary fiber and grape skin soluble dietary fiber, fed After 50 d, the blood was collected and the content of short-chain fatty acids (SCFA) in serum of rats with lipid-lowering test was measured by hollow fiber supported liquid membrane extraction-gas chromatography. The effects of dietary fiber with different grapes on high fat diet Effect of rat serum SCFA. The results showed that compared with the MC group, the ability of acid production of acetic acid, isobutyric acid and isovaleric acid was increased in G1 (ultrafine pulverization) group with the increasing levels of 76.07%, 194.51% and 10.27%, respectively. For propionic acid , Butanoic acid and pentanoic acid, the inhibition levels were 36.58%, 17.68% and 22.79% respectively. G2 inhibited the production of each acid by 44.27% (acetic acid), 78.54% (propionic acid), 60.47% (butyric acid), 25.43% (isobutyl Acid) and 24.84% (isovaleric acid). GS (soluble dietary fiber) had an increase of acetic acid production capacity of 127.79%, but inhibited other acids with the inhibitory levels of 44.76%, 35.37% and 66.58%, respectively Butyric acid), 39.32% (isovaleric acid) and 63.84% (valeric acid).