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为了解鳜(Siniperca chuatsi)食用含有孔雀石绿(malachite green,MG)的饵料鱼后,其体内MG及其代谢物无色孔雀石绿(leucomalachite green,LMG)的残留消除规律,以期为孔雀石绿的监管提供基础数据,本实验模拟了自然养殖条件,对饲养于池塘网箱中的鳜连续投喂10 d经1 mg/L MG溶液浸泡2 min后的鲮(Cirrhinus molitorella),投喂量为5%(m/m),于停药后0、6、12 h及1、3、6、10、15、20、30、40、50、70、90、120、150和180 d采集鳜肌肉样品,各采集点随机取6尾以上鳜,采用液相色谱串联质谱法测定鳜肌肉中MG和LMG的残留量。结果发现,阳性饵料鱼投喂结束后,鳜体内未检出MG,LMG残留浓度也较低,在0 h鳜肌肉中LMG的浓度为8.62μg/kg,随后缓慢降低,10 d时鳜肌肉中检测不到LMG。本研究表明,养殖过程中,阳性饵料鱼体内的MG会通过食物链传递给鳜,造成鳜体内LMG检出,建议执法部门对鳜的饵料鱼进行监控,以确保鳜的食用安全。
In order to understand the rule of residual elimination of MG and its metabolite leucomalachite green (LMG) in the feed of Siniperca chuatsi containing malachite green (MG) Green regulation provided the basic data. In this experiment, we simulated the conditions of natural culture. The feeding of Cirrhinus molitorella in the pond cages was continued for 10 days after immersed in 1 mg / L MG solution for 2 min, Was 5% (m / m) and collected at 0, 6, 12 h and 1, 3, 6, 10, 15, 20, 30, 40, 50, 70, 90, 120, 150 and 180 d Muscle samples were collected randomly from each collection point to more than 6 鳜, and the residues of MG and LMG in muscle were determined by liquid chromatography-tandem mass spectrometry. The results showed that MG and LMG were not detected in the body after the feeding of the positive bait fish, and LMG concentration was 8.62 μg / kg in the muscle at 0 h and then decreased slowly. After 10 d, LMG not detected. This study shows that in the process of breeding, the MG in the positive bait fish will be transmitted to the fish through the food chain, resulting in the detection of LMG in fish body. It is suggested that law enforcement agencies should monitor the fish bait to ensure the food safety.