论文部分内容阅读
目的 克隆与肿瘤转移抑制相关的基因 ,探讨成骨肉瘤转移的分子机理。方法 采用抑制性消减杂交技术构建了人成骨肉瘤低转移细胞株 SOSP- 96 0 7的消减文库 ,对部分克隆进行了测序和同源性分析 ,用 Northern杂交及反转录 PCR技术证实了克隆的表达情况。结果 在低转移细胞株 SOSP- 96 0 7中高表达的克隆中有 2个克隆与人端粒结合因子 2 (telomeric repeat binding factor2 ,TERF2 )同源。Northern杂交及反转录 PCR证实这个基因只在低转移细胞中表达 ,而在高转移人成骨肉瘤细胞株 SOSP-M和裸鼠肺转移结节中均未表达。结论 TERF2可能在维持肿瘤细胞自身相对稳定、抑制骨肉瘤演进中起重要作用。
Objective To clone the genes related to tumor metastasis inhibition and explore the molecular mechanism of osteosarcoma metastasis. Methods Suppression subtractive hybridization was used to construct a subtractive library of human osteosarcoma low metastatic cell line SOSP-96 0 7. Some of the clones were sequenced and their homologies were analyzed. Clones were confirmed by Northern blot and reverse transcription PCR The expression of the situation. Results Two of the clones highly expressed in the low metastatic cell line SOSP-96 0 7 were homologous to telomere repeat binding factor 2 (TERF2). Northern blot and reverse transcription PCR confirmed that this gene was only expressed in low metastatic cells but not in highly metastatic human osteosarcoma cell lines SOSP-M and in nude mice. Conclusion TERF2 may play an important role in maintaining the relative stability of tumor cells and inhibiting the evolution of osteosarcoma.