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目的:观察布地耐德混悬液雾化吸入对哮喘模型肺组织STAT6的干预作用。方法:BALB/C小鼠被随机分为空白对照组、哮喘模型组、激素干预组,除空白对照组外其他各组小鼠在试验第1天、7天和第14天通过腹腔注射0.2 m l混有40 mg氢氧化铝和10μg OVA的pH 7.4 PBS液致敏,在试验第15天用5%的OVA对小鼠进行雾化激发,连续1周,激素干预组在激发的同时给予布地耐德混悬液(1 mg)雾化吸入1周。最后1次激发后24 h放血杀死小鼠,检测血浆IgE水平和血中嗜酸细胞计数,留取肺组织标本用10%的甲醛固定,通过免疫组化的方法对各组小鼠肺组织STAT6的表达进行检测。结果:布地耐德混悬液雾化吸入明显抑制了哮喘模型肺组织STAT6的表达,阻止了哮喘模型鼠气道过敏性炎症。结论:布地耐德混悬液雾化吸入不但可以减轻哮喘模型气道的过敏性炎症,同时可以通过抑制肺组织STAT6的表达进而抑制过敏性炎症的信号传导系统。
OBJECTIVE: To observe the effect of inhalation of Budesonide suspension on STAT6 in lung tissue of asthmatic model. Methods: BALB / C mice were randomly divided into blank control group, asthma model group and hormone intervention group. All the other groups except the blank control group were injected intraperitoneally with 0.2 ml intraperitoneal injection on days 1, 7 and 14 The mice were sensitized with 40 mg of aluminum hydroxide and 10 μg of OVA in pH 7.4 PBS. Mice were challenged with 5% OVA on the 15th day of the experiment for 1 week. The hormone intervention group was given budesonide Germany suspension (1 mg) inhalation inhalation for 1 week. After the last challenge, mice were sacrificed at 24 h after bloodletting, plasma IgE level and blood eosinophil count were measured. Lung tissue samples were collected and fixed with 10% formaldehyde. Immunohistochemistry was used to detect the lung tissue STAT6 expression was detected. RESULTS: Inhalation of budesonide suspension significantly inhibited the STAT6 expression in lung tissue of asthmatic model and prevented airway allergic inflammation in asthmatic model rats. Conclusion: Budesonide suspension inhalation can not only reduce the allergic inflammation in asthmatic model airway, but also inhibit the signal transduction system of allergic inflammation by inhibiting the expression of STAT6 in lung tissue.