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目的:研究塞络通胶囊(塞络通)对多发梗死性痴呆(multi-infarct dementia,MID)大鼠学习记忆功能的改善作用及其机制。方法:SD大鼠随机分为假手术组、模型组、阳性药组以及塞络通高、低剂量组。采用SD大鼠颈内动脉注射微球血管栓塞剂的方法建立多发梗死性痴呆模型;手术成功后第10天,大鼠分别灌胃给予蒸馏水、盐酸美金刚(20 mg.kg-1)以及高、低剂量的塞络通(16.5,33.0 mg.kg-1),每日1次,连续60 d;检测塞络通不同剂量对模型大鼠Morris水迷宫潜伏期、路径长度,以及脑组织胆碱乙酰转移酶(ChAT)和乙酰胆碱酯酶(AchE)活性的影响。结果:与假手术组比较,多发梗死性痴呆大鼠在Morris水迷宫实验中的潜伏期及路径长度均明显延长(P<0.05或P<0.01),脑组织ChAT活性明显降低(P<0.05);塞络通干预60 d后明显缩短模型大鼠在Morris水迷宫实验中的潜伏期及路径长度(P<0.05或P<0.01);并且明显降低脑组织AchE活性而升高ChAT活性(P<0.05或P<0.01)。结论:塞络通对多发梗死性痴呆大鼠认知功能障碍具有一定程度的改善作用,作用机制可能与其对脑组织ChAT活性以及AchE活性不同的调节相关。
Objective: To study the effect and mechanism of “Cereuluotong capsule” on learning and memory in multi-infarct dementia (MID) rats. Methods: SD rats were randomly divided into sham-operation group, model group, positive drug group and high-dose and low-dose group. A rat model of multi-infarct dementia was established by injecting microspheres with vascular embolization into the internal carotid artery of SD rats. On the 10th day after the operation, rats were administered intragastrically with distilled water, memantine hydrochloride (20 mg.kg-1) and high (16.5, 33.0 mg.kg-1) once daily for 60 days. The effects of different doses of plug on the Morris water maze latency, path length, Acetyltransferase (ChAT) and acetylcholinesterase (AchE) activity. Results: Compared with the sham - operated group, the latency and path length of Morris water maze test in rats with multi - infarct dementia were significantly prolonged (P <0.05 or P <0.01), and the activity of ChAT in brain decreased significantly (P <0.05). After 60 days of extubation, the latency and path length (P <0.05 or P <0.01) of Morris water maze test in model rats were significantly shortened, and the activity of AchE in brain tissue was increased significantly (P <0.05 or P <0.01). Conclusion: Celuotong can ameliorate the cognitive dysfunction in rats with multi-infarct dementia. The mechanism may be related to the different regulation of ChAT activity and AchE activity in brain.