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目的改变红细胞电荷性质,克服因红细胞与单链DNA均带负电荷而引起的排斥力,达到单链DNA易与红细胞靠近并特异结合的目的。方法以不改变红细胞电荷性质的生理盐水及低离子强度液反应介质为对照组,使用凝聚胺溶液反应介质为试验组,在室温下与ss DNA孵育30 min,并定量检测各组结合于红细胞的单链DNA拷贝数。结果在生理盐水、LISS液、凝聚胺溶液反应介质中,与红细胞结合的单链DNA拷贝数分别为(4.22±2.17)×10~7/μL、(6.13±5.50)×10~7/μL、(8.54±2.97)×10~(10)/μL(n=9)。试验组与对照组间比较P<0.05。结论在凝聚胺溶液反应介质中,携带负电荷的ssDNA可更好地与红细胞结合。与对照组相比,试验组中结合于红细胞的单链DNA拷贝数量级数可提高10~3拷贝/μL。
Objective To change the nature of the charge of erythrocytes and overcome the repulsion caused by the negative charge of erythrocytes and single-stranded DNA, so that single-stranded DNA can easily approach erythrocytes and bind specifically. Methods The normal saline and low ionic strength reaction medium without changing the charge properties of erythrocytes were used as the control group. The reaction medium of polybrene solution was used as the experimental group and incubated with ss DNA at room temperature for 30 min. The binding of RBCs to erythrocytes Single-stranded DNA copy number. Results The copy number of single stranded DNA that bound to erythrocytes was (4.22 ± 2.17) × 10 ~ (7) / μL, (6.13 ± 5.50) × 10 ~ 7 / μL in physiological saline solution, LISS solution and polybrene solution, (8.54 ± 2.97) × 10 ~ (10) / μL (n = 9). The experimental group and control group, P <0.05. CONCLUSIONS In the polybrene solution reaction medium, ssDNA carrying negative charges can bind to erythrocytes better. Compared with the control group, the number of single-stranded DNA copies bound to erythrocytes in the test group can be increased by 10 to 3 copies / μL.