论文部分内容阅读
目的为进一步摸清O157∶H7大肠杆菌在本地区动物携带情况,于2005～2008年采集金乡县部分农家动物粪便共计1654份。方法用免疫磁珠富集方法捕获O157∶H7,接种于山梨醇—麦康凯培养基,可疑菌落转种科玛嘉大肠杆菌O157∶H7显色培养基之后进行生化和血清学鉴定。结果4年从牛、鸡、猪、羊等动物粪便中共检出O157∶H7大肠杆菌59株,检出率为3.57%,其中牛携带率最高为8.02%,结论提示本地区有肠出血性大肠杆菌O157:H7感染的可能,应加强动物管理和疫情监测,防止疫情扩散蔓延。
Objective To further clarify the O157: H7 Escherichia coli in the region of animal carrying, collected in 2005 ~ 2008 part of Jinxiang County farm animal excrement a total of 1654. Methods O157: H7 was captured by immunomagnetic beads enrichment method and inoculated into sorbitol - McCassay culture medium. The suspicious colonies were transformed into Kemaga E. coli O157: H7 medium and then biochemically and serologically identified. Results A total of 59 strains of O157: H7 Escherichia coli were detected from the faeces of cattle, chickens, pigs and sheep in the past 4 years. The detection rate was 3.57%, of which the highest rate of cattle carrying was 8.02%. The conclusion suggested that there were intestinal hemorrhagic large intestine Bacteria O157: H7 infection may be animal management and epidemic prevention should be strengthened to prevent the spread of the spread of the epidemic.