柑橘和土壤中四螨嗪残留量的液相色谱测定法

• 作 者: 谭建兵 周亮 魏艳艳
• 机 构: 重庆市开县疾病预防控制中心,
• 出 处: 职业与健康
• 发表日期: 2013年18期
• 关键词: 四螨嗪 残留量 农药残留测定 精密度试验 平底烧瓶 分液漏斗 硅胶柱 最大残留限量 石油醚 甲醇定容

目的建立用液相色谱测定柑橘和土壤中四螨嗪的农药残留量的方法。方法样品用丙酮提取,干装硅胶柱净化,利用HP 1100高效液相色谱进行检测。结果四螨嗪农药的检出限为0.01 mg/kg,土壤的添加回收浓度在0.05～2.01mg/kg时,平均回收率为92.1%～95.9%,相对标准偏差为0.30%～5.34%,柑橘的添加回收浓度在0.05～2.01 mg/kg时,平均回收率为92%～95.4%,相对标准偏差为0.33%～4.60%。结论该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求。

Objective To establish a method for the determination of pesticide residues in tetracalcium in citrus and soil by liquid chromatography. Methods The samples were extracted with acetone and cleaned on a silica gel column. The samples were detected by HP 1100 high performance liquid chromatography. Results The detection limit of the four moxide pesticides was 0.01 mg / kg. The average recoveries were 92.1% ~ 95.9% and the relative standard deviations (RSDs) were 0.30% ~ 5.34% when the concentration was 0.05 ~ 2.01 mg / kg. The average recoveries ranged from 92% to 95.4% with the relative standard deviations from 0.33% to 4.60% at 0.05 ~ 2.01 mg / kg. Conclusion The sensitivity, accuracy and precision of this method are in line with the technical requirements of pesticide residue determination.