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A new method for the determination of antithrombotic activity of egg white protein hydrolysate(EWPH)was developed using amicroplate reader.Reaction was carried out at 37℃ and pH 7.2 with fibrinogen concentration 0.1%.Microplate reading wasconducted at 405 nm.Inhibition rate of EWPH on thrombin activity showed linearity(R~2=0.9971),when the inhibition rate was inthe range of 10-90%.The lower limit of detection(LLD,at 99.7%probability)and the biological limit of detection(BLD,at 99.7%probability)of the method were 10.643 and 40 mg/mL,respectively.The repeatability standard deviation(R.S.D.)was 1.08%.Thestandard deviation of the method was 0.027 AT-U.
A new method for the determination of antithrombotic activity of egg white protein hydrolysate (EWPH) was developed using amicroplate reader. Reaction was carried out at 37 ° C and pH 7.2 with fibrinogen concentration 0.1%. Microplate reading wasconducted at 405 nm. Inhibition rate of EWPH on thrombin activity showed linearity (R ~ 2 = 0.9971) when the inhibition rate was inthe range of 10-90%. The lower limit of detection (LLD, at 99.7% probability) and the biological limit of detection (BLD, at 99.7 % probability) of the method were 10.643 and 40 mg / mL, respectively. The repeatability standard deviation (RSD) was 1.08%. The standard deviation of the method was 0.027 AT-U.