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目的 : 探讨铅对海马长时程增强 (LTP)的影响与海马不同亚区一氧化氮合酶(NOS)变化的关系以及锌的拮抗作用。方法 : 采用反映学习记忆功能的 Y迷宫法测试大鼠神经行为的改变 ;用 NADPH-黄递酶 (NADPH- d)组化法和神经元型 NOS(n NOS)的免疫组化法研究大鼠海马不同亚区 NOS的活性及表达情况。结果 : 染铅组大鼠的学习记忆能力比铅锌组和对照组明显下降 (P<0 .0 5 ) ,铅锌组与对照组之间无差别 ;组化及免疫组化显示 :染铅组大鼠海马CA1区和齿状回的 NOS和 n NOS阳性神经元明显少于铅锌组和对照组 (P<0 .0 5 ) ,在 CA3区无差别 ,铅锌组与对照组各亚区均无差别。结论 : 铅可损伤大鼠学习记忆能力 ,鉴于 NOS参与LTP这一代表学习记忆的电生理指标的形成和维持 ,推测铅对学习记忆和海马 LTP的影响可能与染铅后海马各区 NOS的不同变化有关。锌对铅引起的学习记忆损伤和 NOS的影响有拮抗作用。
AIM: To investigate the relationship between the effects of lead on hippocampal long-term potentiation (LTP) and the changes of nitric oxide synthase (NOS) in different sub-regions of hippocampus and the antagonism of zinc. Methods: The changes of neurobehavioral changes in rats were observed by Y-maze method, which reflected learning and memory function. The expressions of NADPH-d and nNOS were detected by immunohistochemistry Activity and expression of NOS in different subregions of hippocampus. Results: The learning and memory ability of lead-exposed rats was significantly lower than that of lead-zinc and control groups (P <0.05), there was no difference between lead-zinc group and control group. Histochemical and immunohistochemical staining showed that lead NOS and nNOS positive neurons in hippocampal CA1 and dentate gyrus were significantly less than those in lead and zinc group and control group (P <0.05), no difference in CA3 area, District no difference. Conclusion: Lead can impair the ability of learning and memory in rats. In view of the formation and maintenance of electrophysiological index of LTP, which represents LTP, learning and memory, presumably the effect of lead on learning and memory and hippocampal LTP may be related to the changes of NOS in hippocampus related. Zinc on lead-induced learning and memory impairment and the impact of NOS antagonistic effect.