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目的:研究EPHX2 rs1042032(A/G)多态性与长沙地区汉族人群脑梗塞继发性癫痫遗传易感性关联关系。方法:收集中南大学湘雅医院及湘雅三医院脑梗塞未继发癫痫及脑梗塞继发癫痫患者血液标本。利用病例-对照研究方法,将标本分为对照组(即无EP组)402例和病例组(即EP组)124例,提取血样的DNA。然后根据人类基因组DNA序列,设计EPHX2 rs1042032 PCR引物,用PCR方法对目的基因EPHX2进行扩增。通过聚合酶链反应-限制性片段长度多态性(PCR-restriction fragment length polymorphism,PCR-RFLP)方法,先对扩增的目的基因片段进行酶切,然后通过凝胶电泳对不同的等位基因进行分辨。结果:EP组和无EP组间EPHX2 rs1042032多态位点基因型分布无显著统计学差异(P>0.05)。结论:EPHX2 rs1042032的基因多态性与脑梗塞继发性癫痫可能无关。
AIM: To investigate the association between the polymorphism of rs1042032 (A / G) gene and the susceptibility to epilepsy secondary to cerebral infarction in Han nationality in Changsha area. Methods: Blood samples of patients with epilepsy secondary to cerebral infarction and secondary epilepsy after cerebral infarction were collected from Xiangya Hospital and Xiangya Third Hospital of Central South University. Using the case-control study, 402 samples of the control group (ie, no EP group) and 124 cases of the case group (EP group) were collected and the DNA of the blood samples was extracted. Then according to human genomic DNA sequence, EPHX2 rs1042032 PCR primer was designed and the target gene EPHX2 was amplified by PCR method. By PCR-restriction fragment length polymorphism (PCR-RFLP) method, the amplified target gene fragment was digested first, and then different alleles were analyzed by gel electrophoresis To distinguish. Results: There was no significant difference in the genotypes of EPHX2 rs1042032 polymorphism between EP group and non-EP group (P> 0.05). Conclusion: The gene polymorphism of EPHX2 rs1042032 may not be associated with secondary epilepsy after cerebral infarction.