论文部分内容阅读
本研究利用聚丙烯酰胺凝胶电泳法分析了甜瓜(Cucumis melo.L)84—3等六个杂交种及其双亲的苗期过氧化物酶同工酶谱变化,按生长顺序在苗期进行了四次取样,每次取样均取全株。分析结果表明:甜瓜苗期生一片真叶时酶谱丰富,为取样最佳时期,同一组合中,F_1的过氧化物酶同工酶谱与亲本的过氧化物酶同工酶谱是有差异的,存在“杂种酶带”和“互补酶带”,利用过氧化物酶同工酶谱技术对甜瓜84-3等六个组合进行纯度检测结果与田间纯度检测结果相比符合率为99.1％。
In this study, polyacrylamide gel electrophoresis was used to analyze the changes of peroxidase isozymes in six hybrids (Cucumis melo L.) 84-3 and their parents at the seedling stage The four samples, each sample taken the whole plant. The results showed that there were abundant enzyme bands in the leaves of melon at the seedling stage, which was the best sampling period. In the same combination, the peroxidase isozymes of F 1 and the peroxidase isozymes of parents were different , There were “hybrid enzyme band” and “complementary enzyme band”, and the coincidence rate of purity test results of six combinations of melon 84-3 with peroxidase isozyme technology was 99.1% compared with the field purity test results.