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目的研究单侧液压脑损伤(FPI)对大鼠双侧海马区胶质纤维酸性蛋白(GFAP)表达和CA1区突触传递的影响。方法建立大鼠单侧液压脑损伤模型,脑标本分为对照组(包括正常对照和假手术对照)、FPI损伤同侧组和FPI损伤对侧组。免疫组化法检测海马水平切片GFAP表达,对海马CA1区锥体神经元进行细胞内记录。结果FPI大鼠双侧海马齿状回门区和CA1区GFAP表达均比对照组明显增强。FPI损伤同侧组兴奋性输入-输出关系曲线的斜率比其他两组显著增大(P<0.05);FPI损伤同侧组和对侧组双脉冲易化(PPF)比值和抑制性突触后电位(IPSP)幅值均比对照组显著减小(P<0.05);FPI损伤同侧组和对侧组双脉冲抑制(PPD)比值均比对照组显著增大(P<0.05)。结论大鼠单侧液压脑损伤对双侧海马均可产生影响,导致双侧海马CA1区兴奋性突触传递增强,抑制性突触传递减弱。
Objective To investigate the effects of unilateral hydraulic brain injury (FPI) on glial fibrillary acidic protein (GFAP) and synaptic transmission in CA1 area of bilateral hippocampus. Methods Unilateral hydraulic brain injury model was established in rats. Brain samples were divided into control group (including normal control and sham operation control), FPI lesion ipsilateral group and FPI lesion contralateral group. Immunohistochemistry was used to detect the expression of GFAP in hippocampal slices, and the pyramidal neurons in hippocampal CA1 area were recorded intracellularly. Results The expression of GFAP in bilateral hippocampal dentate gyrus and CA1 area in FPI rats was significantly higher than that in control group. Compared with the other two groups, the slope of excitatory input-output curve of ipsilateral FPI lesion was significantly increased (P <0.05); PPF ratio and inhibitory postsynaptic (P <0.05). The PPD ratio in both ipsilateral and contralateral FPI groups was significantly higher than that in control group (P <0.05). Conclusion Unilateral hydraulic brain injury in rats may affect both bilateral hippocampus, resulting in increased excitatory synaptic transmission in hippocampal CA1 region and weakened inhibitory synaptic transmission.