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目的:观察延胡索碱预处理对大鼠缺血-再灌注损伤心肌细胞内Ca2+浓度的调节干预作用。方法:清洁级成年SD大鼠30只,随机分为6组(延胡索碱高、中、低剂量预处理组,经典缺血预处理组,注射用水组和假手术组),结扎左冠状动脉前降支近段30min后再灌注60min后,断头放血,取出心脏,分离心肌细胞,利用流式细胞仪(flow cytometer,FCM)结合Fluo-3染色技术检测心肌细胞内钙离子浓度变化。结果:与假手术组比较,模型组心肌细胞内钙离子平均荧光强度增强(P<0.01);与模型组比较,经典缺血预处理组和延胡索碱高、中、低剂量组心肌细胞内钙离子荧光强度均减弱(P<0.01),而且高剂量组的这种药物预处理保护作用与经典缺血预处理组相当(P>0.05)。结论:延胡索碱预处理可减少心肌细胞内Ca2+浓度,拮抗Ca2+蓄积超载,从而保护心肌缺血-再灌注损伤。
Objective: To observe the effects of pretreatment with corydaline on the regulation of intracellular Ca2 + concentration in ischemia / reperfusion injury in rats. Methods: Thirty adult SD rats of clean grade were randomly divided into 6 groups (high, medium and low dose pretreatment group, classic ischemic preconditioning group, water for injection group and sham operation group), ligation of left anterior coronary artery After 30 minutes of reperfusion, reperfusion 60 minutes later, the blood was decapitated and the heart was removed. Cardiomyocytes were isolated and the changes of intracellular calcium concentration were detected by flow cytometer (FCM) and Fluo-3 staining. Results: Compared with sham operation group, the mean fluorescence intensity of intracellular calcium in model group was significantly increased (P <0.01). Compared with model group, the levels of intracellular calcium (P <0.01), and the protective effect of this drug in the high-dose group was similar to that of the classic ischemic preconditioning group (P> 0.05). CONCLUSION: Pretreatment with fumaric alkali can reduce the intracellular Ca2 + concentration and antagonize the overloading of Ca2 + accumulation, thus protecting myocardial ischemia-reperfusion injury.