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目的:探讨膀胱移行细胞癌(bladder transitional cell carcinoma,BTCC)B7-H1miRNA结合靶点区C/G单核苷酸多态性(single nucleotide polymorphism,SNP)与B7-H1表达的关系。方法:选择BTCC患者80例,男58例,女22例,分别取血液及肿瘤组织标本(设为BTCC组);对照者70例,均为同期健康体检者,男52,女18例,取血液标本(设为对照组)。聚合酶链反应-限制性片段长度多态性分析技术(PCR-RELP)检测血液标本中B7-H1miRNA结合靶点区SNP情况,采用免疫组织化学染色法检测肿瘤组织中B7-H1的表达情况。结果:BTCC组在B7-H1miRNA结合靶点区位点rs4143815上的基因型与对照组相比差异有统计学意义,G突变基因明显增加,其中58例B7-H1表达阳性的BTCC患者中,CC型11例,CG型12例,GG型35例;22例B7-H1表达阴性的BTCC患者中,CC型8例,CG型9例,GG型5例;GG型B7-H1表达阳性率明显高于CC型和CG型,差异均有统计学意义,而CC型与CG型比较,差异无统计学意义。同时,B7-H1miRNA结合靶点区SNP基因型与膀胱癌病理分级和临床分期之间均具有相关性,而与肿瘤大小无明显相关性。结论:BTCC的B7-H1miRNA结合靶点区C/G SNP有可能是B7-H1表达上调进而诱导肿瘤细胞发生免疫逃逸的机制之一。
Objective: To investigate the relationship between B7-H1 mRNA and B7-H1 expression in bladder transitional cell carcinoma (BTCC) and C / G single nucleotide polymorphisms (SNPs). Methods: Eighty patients with BTCC were selected, including 58 males and 22 females. Blood samples and tumor tissues (set as BTCC group) were selected. The control group included 70 healthy subjects, 52 males and 18 females, Blood samples (set as control group). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) was used to detect SNP in B7-H1miRNA binding target region in blood samples. The expression of B7-H1 in tumor tissue was detected by immunohistochemical staining. Results: The genotypes of rs4143815 in B7-H1miRNA binding site of BTCC group were significantly different from those in control group, and the G gene mutation was significantly increased. Of the 58 BTCC patients with positive B7-H1 expression, CC type 11 cases, 12 CG genotypes and 35 GG genotypes. Among 22 BTCC patients with negative B7-H1 expression, 8 were CC, 9 were CG and 5 were GG. The positive rate of GG B7-H1 was significantly higher There were significant differences between CC type and CG type, but there was no significant difference between CC type and CG type. At the same time, SNP genotype of B7-H1miRNA binding target region was correlated with pathological grade and clinical stage of bladder cancer, but not with tumor size. CONCLUSION: B7-H1 mRNA binding to C / G SNP in BTCC may be one of the mechanisms of up-regulation of B7-H1 expression and tumor cell immune escape.