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探讨癫持续状态 (StatusEpilepticus,SE)时细胞凋亡的发生及其与海马硬化的关系。采用锂 匹罗卡品诱发大鼠SE模型 ,在SE的不同时点采大鼠脑标本 ,利用TUNEL染色方法检测大鼠海马皮质神经元的凋亡出现情况。结果发现 ,正常对照组大鼠大脑皮质可见散在的TUNEL阳性细胞 ,海马区未见TUNEL阳性细胞。SE1h ,皮质TUNEL阳性细胞数即开始增加 ,SE后 8h ,海马区开始出现TUNEL阳性细胞 ,SE后 1d ,大脑皮质TUNEL阳性细胞数开始明显增加 ,海马区也可见到较多TUNEL阳性细胞。SE后 5d ,皮质及海马的TUNEL阳性细胞数达到高峰。 7d时皮质及海马TUNEL阳性数均明显下降。结果提示 ,SE可引起神经元凋亡 ,5d时达到高峰 ,7d时已明显下降。神经元凋亡与SE引起的迟发性神经元死亡有关 ,并参与了海马硬化的形成。
To investigate the occurrence of apoptosis in status epilepticus (SE) and its relationship with hippocampal sclerosis. Rat models of SE were induced by lithium pilocarpine, brain samples were taken at different time points of SE, and the apoptosis of hippocampus cortical neurons was detected by TUNEL staining. The results showed that scattered control TUNEL positive cells in the cerebral cortex of rats in normal control group, no TUNEL positive cells in hippocampus. SE1h, the number of TUNEL-positive cells started to increase. TUNEL positive cells began to appear in hippocampus 8 h after SE. At 1 d after SE, the number of TUNEL-positive cells began to increase in hippocampus and more TUNEL positive cells were seen in hippocampus. At 5 days after SE, the number of TUNEL positive cells in cortex and hippocampus peaked. On the 7th day, the number of TUNEL positive cells in cortex and hippocampus decreased significantly. The results suggest that SE can cause neuronal apoptosis, reached the peak on the 5th day and decreased significantly on the 7th day. Neuronal apoptosis is associated with delayed neuronal death caused by SE and is involved in the formation of hippocampal sclerosis.