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目的：用糖皮质激素（ＧＣ）制造大鼠“阳虚”模型，以探讨其体内代谢变化。方法：１６只３月龄雄性ＳＤ大鼠，体重３４５～３４７ｇ，随机分为对照组和激素组，后者喂醋酸泼尼松４．５ｍｇ／ｋｇ，２次／周。处死大鼠前第１０天和第２天，分别给其皮下注射四环素（２５ｍｇ／ｋｇ）和Ｃａｌｃｅｉｎ（５ｍｇ／ｋｇ），以标明分两次注射标记物期间骨形成的情况。３个月后，用２％戊巴比妥钠（４０～５０ｍｇ／ｋｇ）腹腔麻醉并用注射器穿右心室抽血处死，血液送附院血液病研究室进行检测；取下的内脏精心剥离干净，湿组织称重；取胫骨上段用甲基丙烯酸甲酯行不脱钙骨包埋，制成２０μ的薄片。血清碱性磷酸酶（ＡＫＰ）、Ｃａ、Ｐ、Ｋ、Ｃｌ按临床生化学检验法检测：骨片检测为半自动图象数字化分析仪，对胫骨上段松质骨的骨组织作静态和动态测量及计算。结果：与对照组比较，激素组体重减少了８．８３％，内脏重量减轻，其中肾上腺和睾丸分别减少了１２．１２％（Ｐ＜０．０１）和１３．６６％（Ｐ＜０．０５）；血清生化指标ＡＫＰ降低了２７．６３％（Ｐ＜０．０５）；胫骨上段骨小梁面积及数量分别减少３８％（Ｐ＜０．０５）和３７％（Ｐ＜０．０５），从而出现骨量丢失。结论：本文初步认为阳虚时?
OBJECTIVE: To establish the rat model of “yang deficiency” with glucocorticoid (GC) to explore its metabolic changes in vivo. Methods: Sixteen 3-month-old male Sprague-Dawley rats weighing 345-3 347 g were randomly divided into control group and hormone group, and the latter was fed with 4.5 mg / kg prednisone acetate twice daily. Ten days and two days prior to sacrifice, tetracycline (25 mg / kg) and Calcein (5 mg / kg) were each injected subcutaneously to indicate bone formation during two injections of the marker. Three months later, the rats were sacrificed by intraperitoneal anesthesia with 2% pentobarbital sodium (40-50 mg / kg) and a right ventricle blood pump with a syringe. The blood was sent to the Institute of Hematology Laboratory for testing. The removed viscera was carefully stripped cleanly, Wet tissue weighed; upper tibia take methyl methacrylate non-decalcified bone embedded, made of 20μ sheet. Serum alkaline phosphatase (AKP), Ca, P, K and Cl were detected by clinical biochemical test. The bone chip was detected by semi-automatic digital image analyzer. The static and dynamic measurement of bone tissue in the upper tibial cancellous bone and Calculate. Results: Compared with the control group, the body weight of the hormone group decreased by 8.83% and the visceral weight decreased, while the adrenal gland and the testicle decreased by 12.12% (P <0.01) and 13.66% (P <0.05) ); AKP decreased by 27.63% (P <0.05); the area and number of trabecular bone in the upper tibia decreased by 38% (P <0.05) and 37% (P < Resulting in loss of bone mass. Conclusion: This article initially considered Yang when?