论文部分内容阅读
作者采用自行设计的方法，首次在鼻咽癌病人血清中检测出凝集因子（ＡＦ）。鼻咽癌病人血清以Ｄ－Ｈａｎｋｓ缓冲液倍比稀释（１：２０～２５６０）。人鼻咽癌细胞株（ＣＮＥ－２）经胰蛋白酶消化，戊二醛固定制成单个细胞悬液。两者混合于９６孔培养板，５０００细胞／孔，经３７℃、４℃、再３７℃处理后，在倒置显微镜下观察，以能形成一个≥２０细胞的凝集团块为ＡＦ阳性，结果发现２３１例鼻咽癌病人血清都是阳性，几何平均滴度（ＧＭＴ）１：２６５．１。１１１名正常人中７４人ＡＦ阴性，３７人低滴度阳性（１：２０～１６０），ＧＭＴＩ：３９．２。提示鼻咽癌病人血清ＡＦ水平明显高于正常人（Ｐ＜０．００１）。作者对结果及方法进行了讨论。
The authors used a self-designed method for the first time detected in the serum of patients with nasopharyngeal carcinoma agglutination factor (AF). Nasopharyngeal carcinoma patients were serially diluted in D-Hanks buffer (1: 20-2560). Human nasopharyngeal carcinoma cell line (CNE-2) was trypsinized and fixed with glutaraldehyde to make a single cell suspension. The two were mixed in 96-well culture plates, 5000 cells / well, after 37 ℃, 4 ℃, then 37 ℃ under the inverted microscope to observe, in order to form a ≥ 20 cell agglutination mass was AF positive, found that 231 cases of nasopharyngeal carcinoma patients were all seropositive, the geometric mean titer (GMT) 1: 265.1.111 74 normal AF negative, 37 low-titer positive (1:20 ~ 160), GMTI: 39.2. Prompt nasopharyngeal cancer patients serum AF levels were significantly higher than normal (P <0.001). The authors discussed the results and methods.