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目的通过检测心跳骤停犬复苏后及纳洛酮干预后脑组织中S100B蛋白mRNA的表达情况,了解纳洛酮对脑复苏的影响。方法18只健康杂种犬,随机分成三组,每组6只,予体外电击诱发室颤,对照组:心跳骤停后予标准心肺复苏术;纳洛酮组:心跳骤停后予标准心肺复苏术加纳洛酮;空白组:不诱发室颤,于复苏后6h取脑海马组织行脑形态学检查及S100B蛋白mRNA表达的测定。结果纳洛酮组S100B蛋白mRNA表达明显低于对照组(p<0.01),纳洛酮组脑组织的病理损害低于对照组。结论使用纳洛酮后心肺复苏犬脑组织的病理损害有所减轻,脑组织S100B蛋白的表达显著减少,纳洛酮可能通过减少S100B蛋白的表达而减轻心肺复苏后脑的再灌注损伤。
Objective To investigate the effect of naloxone on cerebral resuscitation by detecting the expression of S100B mRNA in brain tissue after cardioversion and after naloxone intervention. Methods Eighteen healthy mongrel dogs were randomly divided into three groups with 6 rats in each group, which were induced by extracorporeal shock. The control group was given standard cardiopulmonary resuscitation after cardiac arrest. The naloxone group was given standard cardiopulmonary resuscitation Surgery plus naloxone; blank group: does not induce ventricular fibrillation, brain tissue was taken 6 h after the recovery hippocampus brain morphology and S100B protein mRNA expression was measured. Results The expression of S100B mRNA in naloxone group was significantly lower than that in control group (p <0.01). The pathological changes of brain tissue in naloxone group were lower than those in control group. Conclusion The pathological changes of brain tissue after Naloxone treatment are alleviated. The expression of S100B protein in brain tissues is significantly reduced. Naloxone may reduce the reperfusion injury of brain after cardiopulmonary resuscitation by decreasing the expression of S100B protein.