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目的:研究以减毒鼠伤寒沙门菌为载体构建的幽门螺杆菌(Helicobacterpylori,Hp)疫苗的免疫保护机制。方法:将表达不同Hp抗原的减毒鼠伤寒沙门菌(Salmonellatyphimurium)分别免疫小鼠,免疫4周后以Hp攻击;在攻击前及攻击后5周分批处死小鼠,比较各组小鼠Hp定植密度及各项免疫指标的变化。结果:(1)和NS(Normalsalin,NS)对照组相比各免疫组的Hp定植密度显著下降。(2)和NS组相比攻击前后各免疫组血清IgG2a和胃黏膜Th1型细胞因子表达显著升高。(3)和NS组相比攻击后免疫组鼠胃黏膜出现明显的炎症反应。结论:以减毒鼠伤寒沙门菌为载体构建的重组幽门螺杆菌疫苗在小鼠体内诱导出以Th1反应为主并伴随免疫后胃炎的保护性免疫应答。
Objective: To study the protective mechanism of Helicobacter pylori (Hp) vaccine with attenuated Salmonella typhimurium as carrier. Methods: Salmonella typhimurium was immunized with Salmonella typhimurium expressing different Hp antigens and challenged with Hp 4 weeks after challenge. Mice were killed in batches before challenge and 5 weeks after challenge. Hp Planting Density and Various Immune Indexes. Results: (1) The density of Hp colonization significantly decreased in each group compared with the normal control group (NS). (2) The serum IgG2a and the expression of Th1 type cytokines in gastric mucosa were significantly higher than those in NS group before and after challenge. (3) Compared with NS group, there was a significant inflammatory reaction in gastric mucosa of challenge group after challenge. CONCLUSION: Recombinant Helicobacter pylori vaccine constructed with attenuated Salmonella typhimurium as a vector induces a protective immune response in mice that is predominantly Th1-responsive with post-immunization gastritis.