论文部分内容阅读
目的:探讨蝙蝠葛酚性碱对大鼠局灶性脑缺血-再灌注损伤皮层和海马钙蛋白酶活性的影响。方法:采用大鼠大脑中动脉栓塞2 h后,再灌注24 h,即局灶性脑缺血-再灌注损伤模型,用吸光光度法观察缺血侧和非缺血侧皮层与海马钙蛋白酶活性的变化,并探讨蝙蝠葛酚性碱对其活性的影响。结果:大鼠局灶性脑缺血-再灌注后,缺血侧皮层和海马钙蛋白酶活性均显著升高,蝙蝠葛酚性碱(10,20 mg·kg-1)能依剂量降低缺血侧皮层和海马的钙蛋白酶活性。结论:蝙蝠葛酚性碱能降低缺血-再灌注损伤大鼠缺血侧皮层和海马钙蛋白酶的活性,可能是其减轻脑缺血损伤所致神经细胞凋亡或死亡的机制之一。
Objective: To investigate the effects of PAMB on cortical and hippocampus calpains after focal cerebral ischemia-reperfusion injury in rats. Methods: The focal cerebral ischemia-reperfusion injury model was induced by middle cerebral artery occlusion (2 h) and reperfusion (24 h) in rats. The calpain activity in the ischemic and non-ischemic cortex and hippocampus were observed by absorbance spectrophotometry , And to explore the effect of DAPP on its activity. RESULTS: After focal cerebral ischemia-reperfusion, the activity of calpain in the ischemic cortex and hippocampus were significantly increased, and that of mefenoxuron (10,20 mg · kg-1) Calpain activity in the cortex and hippocampus. CONCLUSION: It suggests that it may be one of the mechanisms by which it reduces the apoptosis or death of neurons induced by cerebral ischemic injury in ischemic cortex and hippocampus after ischemia-reperfusion injury.