Identification of stable normalization genes for quantitative real-time PCR, in porcine articular ca

• 作 者: Ryan S McCulloch Melissa S Ash
• 机 构: JointDepartmentofBiomedicalEngineering,UniversityofNorthCarolina,AnimalScienceDepartment
• 出 处: 畜牧与生物技术杂志：英文版
• 发表日期: 2012年4期
• 关键词: 基因鉴定 关节软骨 稳定性 实时定量 Β-肌动蛋白 正常化 PCP 琥珀酸脱氢酶 Cartilage Housekeeping Normalization
• 基金项目: Acknowledgements The authors would like to acknowledge Neese sausage for providing the porcine patellae used in this study. This work was funded by NIAMS/NIH grant 1 R03 AROS4557-01A1.

Background: Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results. The purpose of this study was to identify the most stable house