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培养的自体人表皮细胞移植在理论上讲无排斥反应,但有实验证实培养中培养液附加的胎牛血清(FBS)蛋白能结合到人表皮细胞的膜上,这些异种蛋白会诱发接受移植病人的免疫反应,导致移植物失活和下一次移植的排异反应。表皮细胞的无血清培养方法已有成功的描述,但许多无血清培养基中需加牛脑垂体提取液(BPE),BPE是否象FBS那样会诱发免疫反应?为此,开展了此研究。以人血清和牛BSA作为对照,用 SDS-聚丙烯酰胺凝胶电泳(SDS-
Cultured autologous human epidermal cell transplantation in theory, no rejection, but experiments confirmed that culture medium added fetal bovine serum (FBS) protein can bind to human epidermal cell membrane, these xenogenic proteins will be induced transplant patients Of the immune response, leading to graft rejection and the rejection of the next transplant. Serum-free culture of epidermal cells has been described successfully, but in many serum-free media, bovine pituitary extract (BPE) is required and does BPE induce an immune response like FBS? To this end, we performed this study. Human serum and bovine BSA as a control, SDS-polyacrylamide gel electrophoresis (SDS-