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目的 从细胞骨架角度探讨肝癌细胞粘弹性改变的基础 ,及细胞骨架结构干扰剂对其改变的影响。方法 采用微管吸吮技术测定正常肝细胞和肝癌细胞的粘弹性 ,三参数标准线性固体模型拟合实验结果。结果 肝癌细胞的弹性系数较肝细胞增高 ,秋水仙素、长春花碱使癌细胞粘弹性系数与肝细胞相比呈现不同的效应方式和强度 ,细胞松驰素D则使 2种细胞粘弹性系数均呈现相似的显著下降趋势。结论 细胞骨架结构的完整性是维持肝细胞和肝癌细胞粘弹性及被动变形机能的必要条件 ,2种细胞的粘弹性对细胞骨架干扰剂的效应差异 ,反映了肝癌细胞在细胞骨架结构和机能状态中的变化 ,这种变化可能影响癌细胞的浸润、转移特性以及与微观力学环境的相互作用。
Objective To investigate the basis of changes in viscoelasticity of hepatocellular carcinoma cells from the perspective of cytoskeleton and the effect of cytoskeletal structure interfering agents on their alteration. Methods The viscoelasticity of normal liver cells and hepatocellular carcinoma cells was determined by micropipette suction technique. Three-parameter standard linear solid model was used to fit the experimental results. Results The elasticity coefficient of hepatocellular carcinoma cells was higher than that of hepatocytes. Colchicine and vinblastine made the viscoelastic coefficients of cancer cells show different effects and strengths compared with hepatocytes. Cytochalasin D resulted in two kinds of cell viscoelastic coefficients. Both showed similar significant declines. Conclusion The integrity of the cytoskeletal structure is a necessary condition for maintaining the viscoelasticity and passive deformability of hepatocytes and hepatocellular carcinoma cells. The effects of the viscoelasticity of the two types of cells on cytoskeletal interfering agents are different and reflect the cytoskeletal structure and function of the hepatoma cells. Changes in the medium may affect the infiltration and metastasis of cancer cells and their interaction with the micromechanical environment.