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背景:成鼠癫痫持续状态(statusepilepticus,SE)后出现海马CA1区、CA3区及齿状回广泛的神经元脱失和苔藓纤维发芽已成为大量实验的共识,但有关幼鼠该方面研究得出的结论不一。目的:观察幼鼠致痫后海马区神经元的组织病理学改变。设计:随机对照实验研究。地点和对象:实验地点:北京大学人民医院实验动物中心进行。健康生后15～20dWistar幼鼠54只。随机分成3组,每组18只。生理盐水对照组,地西泮干预组,实验组。每组中用于光镜检查、电镜检查和Timm染色各6只。干预:实验组幼鼠采用氯化锂-毛果芸香碱腹腔注射制成幼鼠癫痫持续状态模型,生理盐水对照组以相同液体量的生理盐水取代毛果芸香碱。地西泮干预组在给予毛果芸香碱30min前给予地西泮腹腔注射。在光镜下观察海马结构的形态学改变。透射电镜下进行超微结构观察。Timm法观察苔藓纤维发芽情况。主要观察指标:①各组大鼠海马区的神经元形态学改变。②各组大鼠超微结构改变。③各组大鼠苔藓纤维发芽情况。结果:实验组CA1区和CA3区及齿状回可见许多神经元发生变性和固缩性坏死。CA2区未见明显改变。电镜下海马区神经元胞体浓缩变性,粗面内质网增生,内质网的核糖体脱落,胶质细胞可见有空泡变性。生理盐水对照组和地西泮干预组无明显的超微结构改变。Timm染色见?
BACKGROUND: Extensive neuronal loss and mossy fiber sprouting in hippocampal CA1, CA3 and dentate gyrus after statusepilepticus (SE) have become the consensus of a large number of experiments. However, Different conclusions. OBJECTIVE: To observe the histopathological changes of neurons in hippocampus after epileptic seizures in young rats. Design: Randomized controlled trial. Location and object: Experimental Location: Peking University People’s Hospital Laboratory Animal Center. 54 healthy Wistar rats aged 15-20 days after birth. Randomly divided into 3 groups, each group of 18. Saline control group, diazepam intervention group, experimental group. Each group for light microscopy, electron microscopy and Timm staining of 6. Intervention: Rats in experimental group were injected intraperitoneally with lithium chloride-pilocarpine to establish the model of status epilepticus in young rats. Physiological saline control group was given the same amount of liquid physiological saline to replace pilocarpine. Diazepam intervention group given pilocarpine 30min given intraperitoneal injection of diazepam. Morphological changes of hippocampal formation were observed under light microscope. The ultrastructure was observed under transmission electron microscope. Timm method to observe the germination of moss fibers. MAIN OUTCOME MEASURES: Neuronal morphological changes in hippocampus of rats in each group. ② ultrastructural changes in rats in each group. ③ Sprouting of mossy fibers in rats in each group. Results: In the experimental group, many neurons showed degeneration and collapsible necrosis in CA1, CA3 and dentate gyrus. CA2 area no significant change. Under the electron microscope, neurons in the hippocampus were concentrated and denatured, the rough endoplasmic reticulum hyperplasia, the endoplasmic reticulum ribosome shedding, and the degeneration of the glial cells were observed. The saline control group and the diazepam intervention group had no obvious ultrastructural changes. See Timm staining?