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为探讨遗传工程水稻再转化的可能性,对其离体子房进行了培养,从子房诱导出愈伤组织并分化成苗.观察结果表明,培养基合 5 比 N6 更适宜于遗传工程水稻子房的培养,在合5 和 N6 条件下,遗传工程水稻各品系间的诱导率和分化率存在差异,且均优于受体湘早籼8 号,培养基合5 条件下各品系诱导率和分化率大小依次为 G E R4, G E R3, G E R1,湘早籼8 号;低温(4~5℃)预处理 48 h 或喷6 B A 72 h 后均能促进雌核愈伤化,低温预处理的效果优于6 B A 的影响.
In order to explore the possibility of re-transformation of genetically engineered rice, its ovary was cultured, callus was induced from the ovary and differentiated into seedlings. The results showed that medium 5 and N6 were more suitable for the cultivation of genetic engineering rice ovary. Under the conditions of 5 and N6, the induction rate and differentiation rate of the genetically engineered rice lines were all different The induction rate and differentiation rate of each line under Xiangzaoxian 8 and medium 5 were G E R4, G E R3, G E R1 and Xiangzaoxian 8 at low temperature (4 ~ 5 ℃) pretreatment 48 h or spray 6 B A 72 h can promote the gynogenesis callus, hypothermia pretreatment effect is better than the impact of 6 B A.