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为了解干扰素α（ＩＦＮ－α）联合白细胞介素２（ＩＬ－２）激活骨髓对白血病细胞的体外净化作用。采用细胞培养法和逆转录多聚酶链反应法检测激活的正常骨髓细胞对Ｋ５６２细胞的净化作用。结果发现ＩＬ－２激活３天的骨髓对Ｋ５６２细胞有净化作用。加用ＩＦＮ－α可明显增强这种净化作用，使其对Ｋ５６２细胞的杀伤率可提高０．４５～２．６６对数级。联合ＩＬ－２和ＩＦＮ－α对骨髓ＣＦＵ－ＧＭ和Ｋ５６２细胞无明显抑制作用。结果表明ＩＦＮ－α可增加ＩＬ－２激活骨髓净化Ｋ５６２细胞的能力。
To investigate the in vitro purification effect of interferon α (IFN-α) combined with interleukin 2 (IL-2) on leukemia cells. The purifying effect of activated normal bone marrow cells on K562 cells was detected by cell culture and reverse transcription polymerase chain reaction. As a result, it was found that IL-2 activated bone marrow for 3 days had a purifying effect on K562 cells. Adding IFN-α can significantly enhance the purification effect, so that the killing rate of K562 cells can be increased by 0.45 to 2.66 logarithm. Combined IL-2 and IFN-α had no obvious inhibitory effect on bone marrow CFU-GM and K562 cells. The results indicate that IFN-α can increase the ability of IL-2 to activate bone marrow to purify K562 cells.