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目的探讨应用小干扰核糖核酸(siRNA)干扰组蛋白去乙酰化酶1(HDAC1)的表达,提高人类宫颈癌He La细胞对顺铂(DDP)化疗敏感性的作用与机制。方法合成针对HDAC1的siRNA采用脂质体法转染宫颈癌He La细胞,将其分为siRNA组、阴性siRNA组及对照组。对照组、siRNA组和阴性siRNA组通过DDP处理后分别作为对照+DDP组、siRNA+DDP组和阴性siRNA+DDP组。采用PCR检测HDAC1 mRNA表达,采用流式细胞仪检测细胞凋亡率,采用四甲基偶氮唑蓝(MTT)比色法检测He La细胞对DDP的敏感性。结果与阴性siRNA组及对照组比较,siRNA组He La细胞的凋亡率增加,差异具有统计学意义(P<0.05);和阴性siRNA组及对照组比较,siRNA组HDAC1 mRNA表达量降低,差异具有统计学意义(P<0.05),siRNA+DDP组IC50值低于阴性siRNA+DDP组及对照+DDP组,差异具有统计学意义(P<0.05)。结论 HDAC1 siRNA可抑制HDAC1基因的表达水平,使组蛋白去乙酰化水平增加,提高宫颈癌He La细胞对顺铂的敏感性。
Objective To investigate the effect of small interfering RNA (siRNA) on the expression of histone deacetylase 1 (HDAC1) and the sensitivity of human cervical carcinoma HeLa cells to cisplatin (DDP) chemotherapy. Methods siRNA targeting HDAC1 was transfected into cervical cancer HeLa cells by lipofectamine and divided into siRNA group, negative siRNA group and control group. The control group, siRNA group and negative siRNA group were treated by DDP as control + DDP group, siRNA + DDP group and negative siRNA + DDP group respectively. The expression of HDAC1 mRNA was detected by PCR. The apoptosis rate was detected by flow cytometry. The sensitivity of HeLa cells to DDP was detected by MTT assay. Results Compared with the negative siRNA group and the control group, the apoptosis rate of Hela cells in siRNA group was increased (P <0.05), and the expression of HDAC1 mRNA in siRNA group was lower than that in negative siRNA group and control group (P <0.05). The IC50 value of siRNA + DDP group was lower than that of negative siRNA + DDP group and control + DDP group (P <0.05). Conclusion HDAC1 siRNA can inhibit the expression of HDAC1 gene, increase the level of histone deacetylation, and improve the sensitivity of cervical cancer He La cells to cisplatin.