论文部分内容阅读
在对冈优158、Ⅱ优808、五优308及天丰优316的杂交种子样品进行室内纯度鉴定时,发现不同SSR引物鉴定的纯度结果存在明显差异。为了探讨其原因,将这4个组合的种子样品在海南进行田间种植鉴定,并按单株进行了分子与田间表型的对比分析。结果表明,冈优158、Ⅱ优808、五优308和天丰优316分别用引物RM208、RM264、RM242和RM164鉴定种子纯度时,只能鉴定出不育系(母本)杂株,室内分子鉴定纯度的结果均高于相应的田间纯度鉴定结果 ;而引物RM341、RM297、RM21和RM110不仅能鉴定出不育系(母本)杂株,还能鉴定出串粉株,室内分子鉴定纯度与田间鉴定纯度相当。因此,室内纯度鉴定时应采用多对引物进行检测,以增加识别串粉株的几率。
When the purity of hybrid seed samples of Gangyou 158, Ⅱyou808, Wuyou 308 and Tianfengyou316 were identified in laboratory, the purity results of different SSR primers were significantly different. In order to explore the reason, the seed samples of these four combinations were identified in field planting in Hainan, and molecular and field phenotypes were compared by single plant. The results showed that male sterile lines (maternal plants) were identified when Gangyou 158, Ⅱyou 808, Wuyou 308 and Tianfengyou 316 were respectively identified by primers RM208, RM264, RM242 and RM164, However, the primers RM341, RM297, RM21 and RM110 not only identified the male sterile lines, but also identified the powdery mildew strains. The identification of indoor purity and Field identification of purity. Therefore, the identification of indoor purity should be used on the detection of multiple pairs of primers to increase the probability of identifying powder string.