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目的:荷叶不同萃取部位的总黄酮含量测定。方法:用正丁醇、乙酸乙酯、乙醚对荷叶水提液萃取,以芦丁为对照品,通过UV对其总黄酮含量进行测定。结果:该测定方法回归方程为y=0.0382x+3*10-5,R2=0.9999;重现性、精密度和稳定性较好,RSD分别为0.64、0.87%、1.21%。结论:为荷叶的总黄酮研究提供依据。
Objective: To determine the content of total flavonoids in different extraction sites of lotus leaf. Methods: The aqueous extract of lotus leaf was extracted with n-butanol, ethyl acetate and ether. Rutin was used as the reference substance and its total flavonoid content was determined by UV. Results: The regression equation was y = 0.0382x + 3 * 10-5, R2 = 0.9999. The reproducibility, precision and stability were good. The RSDs were 0.64,0.87% and 1.21% respectively. Conclusion: The study provides the basis for the study of flavonoids in lotus leaf.