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目的:探讨冠状动脉介入治疗(percutaneouscoronoty intervention,PCI)对急性心肌梗死(AMI)患者血小板生成素(Tpo)及受体基因(C-mpLmRNA)水平的影响。方法:采用酶联免疫吸附法(ELLSA)和半定量逆转录聚合酶(RT-PCR)方法测定34例AMI患者Tpo及C-mpLmRNA水平,其中行PCI手术18例,16例未行介入治疗,比较两组各项指标。结果:与正常对照组比较,AMI患者血浆Tpo及C-mpLmRNA发病后逐渐升高,持续3～5d,24h左右最高,3～5d后已下降。发病时Tpo及C-mpLmRNA与心肌酶呈正相关。结论:Tpo及C-mpLmRNA与AMI发病正相关,可反映病程变化。介入治疗可引起Tpo及C-mpLmRNA水平增高。
Objective: To investigate the effects of percutaneous coronary intervention (PCI) on the levels of thrombopoietin (Tpo) and receptor gene (C-mpLmRNA) in patients with acute myocardial infarction (AMI). Methods: The Tpo and C-mpL mRNA levels were determined in 34 patients with AMI by enzyme-linked immunosorbent assay (ELLSA) and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Among them, 18 patients underwent PCI and 16 patients underwent interventional therapy. Compare the two groups of indicators. Results: Compared with the normal control group, plasma Tpo and C-mpL mRNA in AMI patients gradually increased after 3 ~ 5 days and reached the peak at 24h, and decreased after 3 ~ 5 days. On the onset of Tpo and C-mpLmRNA and myocardial enzymes were positively correlated. Conclusion: Tpo and C-mpL mRNA are positively correlated with the incidence of AMI, which can reflect the course of disease. Interventional therapy can cause Tpo and C-mpL mRNA levels increased.