论文部分内容阅读
目的:探讨P450酶系的CYP19和CYP1B1基因多态性与妊娠期肝内胆汁淤积症(ICP)发病的关系。方法:应用聚合酶链反应-限制性片段多态性技术(PCR-RFLP)和等位基因特异性PCR技术(AS-PCR)对100例ICP患者和100例正常对照孕妇CYP19基因第3外显子Rsal酶切多态和CYP1B1基因外显子3密码子432(C-G)多态性进行分析。结果:①对照组CYP19的基因型AA、AG、GG频率分别为30.0%、50.0%、20.0%,ICP组分别为38.0%、45.0%、17.0%,两组比较差异无统计学意义(P>0.05);对照组等位基因A、G频率分别为55.0%、45.0%,ICP组分别为60.5%、39.5%,两组比较差异也无统计学意义(P>0.05)。②对照组CYP1B1的基因型CC、CG频率分别为80.0%、20.0%,ICP组分别为70.0%、30.0%,两组均无突变纯合子(GG),两组比较差异无统计学意义(P>0.05);对照组等位基因C、G频率分别为90%、10%,ICP组分别为85%、15%,两组比较差异也无统计学意义(P>0.0S)。结论:CYP19外显子3基因多态性和CYP1B1外显子3密码子432基因多态性与ICP发病无关。
Objective: To investigate the relationship between polymorphisms of CYP19 and CYP1B1 in P450 enzymes and the incidence of intrahepatic cholestasis of pregnancy (ICP). Methods: The CYP19 gene exon 3 of 100 pregnant women with ICP and 100 normal controls were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele-specific PCR (AS-PCR) Rsal digestion polymorphism and CYP1B1 gene exon 3 codon 432 (CG) polymorphism were analyzed. Results: ① The CYP19 genotype frequencies in the control group were 30.0%, 50.0% and 20.0%, respectively, and those in the ICP group were 38.0%, 45.0% and 17.0%, respectively. There was no significant difference between the two groups (P> 0.05). The frequencies of allele A and G in control group were 55.0% and 45.0% respectively, while those in ICP group were 60.5% and 39.5% respectively. There was no significant difference between the two groups (P> 0.05). ② The genotype frequencies of CYP1B1 in the control group were 80.0% and 20.0%, respectively, and the ICP group was 70.0% and 30.0% respectively. There was no mutant homozygote (GG) in both groups (P> 0.05). There was no significant difference between the two groups > 0.05). The frequencies of allele C and G in the control group were 90% and 10% respectively, while those in ICP group were 85% and 15% respectively. There was no significant difference between the two groups (P> 0.0S). CONCLUSION: CYP19 exon 3 gene polymorphism and CYP1B1 exon 3 codon 432 gene polymorphism have nothing to do with the incidence of ICP.