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目的:检测胃癌组织与非肿瘤胃组织(胃黏膜及肌组织)中表皮生长因子受体-3(HER-3)、丝苏氨酸激酶(Akt)的表达及胃癌组织中HER-3、Akt的表达与临床病理参数及预后的关系。方法:免疫组化ElivisionTM二步法检测84例胃癌及15例非肿瘤胃组织中HER-3、Akt的表达,应用SPSS 13.0分析HER-3、Akt的表达与胃癌患者临床病理特征及预后的关系。结果:胃癌组织中HER-3阳性率为56.0%(47/84),过表达率为21.4%(18/84),Akt阳性率为48.3%(54/84),过表达率为31.0%(26/84)。HER-3的阳性表达与临床分期、远处转移相关(P值分别为0.038和0.008),Akt的阳性表达与分化程度、淋巴结转移相关(P值分别为0.023和0.028),Akt的过表达与临床分期、肿瘤浸润深度、淋巴结转移相关,P值分别为0.014、0.009和0.034。HER-3阴性的总生存时间(OS)显著优于阳性者(P=0.023),Akt低表达的OS、无进展生存率(PFS)均显著优于高表达者,P<0.001。结论:HER-3、Akt的表达与细胞的恶变相关。HER-3、Akt可能参与肿瘤细胞的粘附、增殖、浸润及转移的过程,可作为胃癌预后的预测因子。
Objective: To detect the expression of epidermal growth factor receptor-3 (HER-3) and serine-threonine kinase (Akt) in gastric cancer tissue and non-tumor gastric tissue The relationship between the expression and clinical pathological parameters and prognosis. Methods: The expressions of HER-3 and Akt in 84 gastric cancer tissues and 15 non-tumorous gastric tissues were detected by immunohistochemical ElivisionTM two-step method. The relationship between the expression of HER-3, Akt and the clinicopathological features and prognosis of gastric cancer was analyzed by SPSS 13.0 . Results: The positive rate of HER-3 in gastric cancer was 56.0% (47/84), the overexpression rate was 21.4% (18/84), the positive rate of Akt was 48.3% (54/84) and the overexpression rate was 31.0% 26/84). The positive expression of HER-3 was correlated with clinical stage and distant metastasis (P = 0.038 and 0.008 respectively). The positive expression of Akt correlated with the degree of differentiation and lymph node metastasis (P = 0.023 and 0.028, respectively) Clinical stage, depth of tumor invasion, and lymph node metastasis, with P values of 0.014, 0.009 and 0.034, respectively. The overall survival time (OS) of patients with HER-3 negative was significantly higher than that of patients with positive HER-3 (P = 0.023). The OS of OS was significantly lower than that of patients with high expression of AKT (P <0.001). Conclusion: The expression of HER-3 and Akt is related to the malignant transformation of cells. HER-3, Akt may be involved in tumor cell adhesion, proliferation, invasion and metastasis, which can be used as a predictor of gastric cancer prognosis.