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背景:越来越多的基础研究显示P2RY2基因在心血管系统中起重要的调节作用。已有研究表明,人类P2RY2基因可能与冠状动脉支架置入后再狭窄的发生相关。目的:验证人类P2RY2基因单核苷酸多态性与冠状动脉支架置入后再狭窄的相关性。设计、时间及地点:病例-对照观察,于2006-03/2008-06在新疆医科大学第一附属医院心脏中心完成。对象:纳入冠状动脉支架置入后再狭窄患者32例,另外选择120例既往无心血管系统疾病史的人作为对照组。方法:选择P2RY2基因的5个单核苷酸多态性rs4944831,rs1783596,rs4944832,rs4382936及rs10898909,通过TaqMan单核苷酸多态性基因分型的方法进行基因分型。主要观察指标:分析并比较2组5个单核苷酸多态性基因型频率和等位基因频率的分布,并应用Logistic回归分析方法分析支架置入后再狭窄主要危险因素对结果的影响。结果:支架置入后再狭窄组与正常对照组相比,rs4944831在基因型和显性模型(TG+GG)的分布差异有显著性意义(P=0.039,0.040);rs10898909在隐性模型(AA)和等位基因的分布差异有显著性意义(P=0.022,0.039)。Logistic回归分析显示,TG+GG在2组的差异消失,而AA在2组的差异依然存在(P=0.016)。结论:人类P2RY2基因的rs10898909可作为冠状动脉支架置入后再狭窄的标志,其AA基因型与冠状动脉支架置入后再狭窄的发生有关。
BACKGROUND: A growing body of basic research has shown that P2RY2 plays an important regulatory role in the cardiovascular system. Studies have shown that the human P2RY2 gene may be related to the occurrence of restenosis after coronary stenting. Objective: To verify the association between single nucleotide polymorphism of human P2RY2 gene and restenosis after coronary stent implantation. DESIGN, TIME AND SETTING: The case-control study was performed at the Cardiac Center of the First Affiliated Hospital of Xinjiang Medical University from March 2006 to June 2008. PARTICIPANTS: Thirty-two patients with restenosis after coronary stenting were enrolled, and another 120 patients previously without history of cardiovascular disease were selected as controls. Methods: Five single nucleotide polymorphisms (rs4944831, rs1783596, rs4944832, rs4382936 and rs10898909) of P2RY2 gene were selected and genotyped by TaqMan single nucleotide polymorphism (SNP) genotyping. MAIN OUTCOME MEASURES: The distribution of genotype frequency and allele frequency in five SNPs in two groups were analyzed and compared. Logistic regression analysis was used to analyze the influence of major risk factors of restenosis after stent implantation. Results: There was significant difference in the distribution of rs4944831 between genotype and dominant model (TG + GG) in the restenosis group (P = 0.039, 0.040) after implantation of the stent. Compared with the normal control group, rs10898909 in the recessive model AA) and allele distribution was significantly different (P = 0.022, 0.039). Logistic regression analysis showed that the difference of TG + GG in two groups disappeared, while the difference of AA in two groups still existed (P = 0.016). Conclusion: rs10898909 of human P2RY2 gene can be used as a marker of restenosis after coronary stenting. The AA genotype is associated with the occurrence of restenosis after coronary stent implantation.