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探讨蕲蛇酶溶栓作用机制。方法应用发色底物（Ｓ２３９０）显色法测定大鼠静脉注射蕲蛇酶２和４Ｕ／ｋｇ（３００和６００μｇ／ｋｇ）后５，１５，２５和４５ｍｉｎ血浆中组织型纤溶酶原激活剂（ｔ－ＰＡ）和纤溶酶原激活剂抑制物（ＰＡＩ－１）的活性。结果正常大鼠静脉注射蕲蛇酶后５ｍｉｎ血浆中ｔ－ＰＡ活性即开始升高，１５ｍｉｎ时达到高峰，低和高剂量组ｔ－ＰＡ活性（ＩＵ／ｍｌ）分别由给药前１６１．８１±２８．７８和１８９．１４±７１．３０升至３８９．５６±１４３．１０和４８２．８７±８５．６７，随后活性下降，至４５ｍｉｎ时基本恢复正常。与此同时，ＰＡＩ－１的活性变化不明显。结论蕲蛇酶溶栓作用与其促进ｔ－ＰＡ释放有关。
To investigate the mechanism of viper’s thrombolytic effect. Methods The chromogenic substrate (S2390) colorimetric method was used to detect the changes of tissue plasminogen activator (PAK) in rats after intravenous injection of viper 2 and 5, 15, 25 and 45 minutes after 4U / kg (300 and 600μg / kg) (T-PA) and plasminogen activator inhibitor (PAI-1). Results The plasma t-PA activity began to increase 5 minutes after intravenous injection of viper’s enzyme in normal rats, reached its peak at 15 minutes, and the t-PA activity (IU / ml) in low and high dose groups was increased from 161.81 ± 28.78 and 189.14 ± 71.30 to 389.56 ± 143.10 and 482.87 ± 85.67, respectively. Then the activity decreased and returned to normal after 45 minutes. At the same time, the activity of PAI-1 did not change significantly. Conclusion Acanthamoebae thrombolytic effect and its promotion of t-PA release.