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目的 确定感染率高且稳定的幽门螺杆菌感染动物模型预处理方案。方法 取蒙古沙鼠200只,随机分为3个实验组及1个对照组。实验组沙鼠在断食、水12h后分别应用pH2盐酸、消炎痛+胃复安和50％乙醇3种不同方案进行预处理,对照组用生理盐水处理。此后继续断食、水12h,再灌喂Hp菌液（109cfu/ml）0.5ml/只。共3次,每次间隔12h。最后1次灌喂后2h给食、水。后每隔4周解剖一批动物,每组10只,进行分离培养、ELISA、PCR、快速尿素酶检测和病理切片检查。结果3组实验动物感染率不同,其中50％乙醇组沙鼠感染率相对较高,达到80％,其病理组织学变化与人体相应病变也极为相似。结论50％乙醇组沙鼠预处理方案感染效果最好,可作为Hp感染动物模型的预处理方法。
Objective To determine the high infection rate and stability of H. pylori infection in animal models of pre-treatment programs. Methods 200 Mongolian gerbils were randomly divided into three experimental groups and one control group. The rats in the experimental group were pretreated with 3 different solutions of pH2 hydrochloric acid, indomethacin, metoclopramide and 50% ethanol respectively after being fasted for 12 h, and the control group was treated with normal saline. Continue eating fasting, water 12h, then Hp bacteria solution (109cfu / ml) 0.5ml / only. A total of 3 times, each interval of 12h. 2h after the last feeding, water. After every 4 weeks, a group of animals were dissected, with 10 animals in each group. Isolation, ELISA, PCR, rapid urease test and pathological examination were performed. Results The rates of infection in experimental animals were different in three groups. The infection rate of gerbils in 50% ethanol group was relatively high, reaching 80%. The histopathological changes were also very similar to those in human body. Conclusions The 50% ethanol group has the best infection effect in gerbils pretreatment program, which can be used as a pretreatment method for animal models of Hp infection.